Cloning and Drought Tolerance Analysis of Maize Stress Responsive Gene ZmTPR2

LIU Ming-xin; FENG Yan-ping; SHI Qing-ling; DOU Dan-dan

doi:10.16590/j.cnki.1001-4705.2024.07.017

Seed > 2024 > 43(7): 17-26. > DOI: 10.16590/j.cnki.1001-4705.2024.07.017

LIU Ming-xin, FENG Yan-ping, SHI Qing-ling, DOU Dan-dan. Cloning and Drought Tolerance Analysis of Maize Stress Responsive Gene ZmTPR2[J]. Seed, 2024, 43(7): 17-26. DOI: 10.16590/j.cnki.1001-4705.2024.07.017

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Cloning and Drought Tolerance Analysis of Maize Stress Responsive Gene ZmTPR2

1. He’nan Seed Industry Development Center;
2. Insititute of Crop Germplasm Resources, He’nan Academy of Agritural Sciences

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Received Date: February 16, 2024

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Abstract

Abstract

Repeat four peptide protein（Tetratricopeptide repeat proteins TPR proteins） is a kind of protein that belongs to the spiral repeat protein superfamily member, as a regulatory proteins involved in the chloroplasts almost all of the steps and photosynthesis, and play an important role, However, there are few reports on whether TPR protein is involved in plant response to abiotic threats. In this study, through transcriptome data, a significantly different gene was discovered in maize（Zea mays L.） in response to drought and rehydration processes, which encoded a TPR protein named ZmTPR2. The gene, located on chromosome 3 of maize, encoded 421 amino acids and had three conserved coiled helix domains TRP. In the course of evolution, it was closely related to sorghum. The results of RT-PCR showed that ZmTPR2 gene was a constitutive expression gene, which was expressed in all vegetative organs and reproductive organs such as roots, stems, leaves, male ear and female ear, and the expression level was the highest in young leaves. Through the simulation experiments of drought, high temperature, high salt and nitrogen deficiency stress, it was found that the expression of this gene was significantly up-regulated under the four kinds of stress, and the difference ratio reached a peak of 15.23 times when the drought was 24 hours. The expression level of ZmTPR2 gene in drought tolerant inbred line Zheng58 was significantly higher than that in drought sensitive inbred line B73 under both normal and drought stress treatments, and its expression level was closely related to drought resistance. Arabidopsis thaliana plants overexpressing ZmTPR2 were obtained by genetic transformation. Compared with Col, Arabidopsis thaliana plants overexpressing ZmTPR2 under drought stress had better growth, higher leaf water content and chlorophyll content, lower content of malondialdehyde（MDA）, and stronger activities of superoxide dismutase（SOD） and oxide dismutase（POD）. These results indicated that ZmTPR2 played a positive regulatory role under drought stress.
- maize,
- tetrapeptide repeat protein,
- abiotic stress,
- drought tolerance

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References (26)

References

[1]	Chen Z H,Chen G,Dai F,et al.Molecular evolution of grass stomata[J].Trends Plant Sci,2017,22(2):124-139.
[2]	胡伟,颜彦,韦运谢,等.小麦TaNAC5基因克隆及表达分析[J].西北植物学报,2014,34(12):2394-2398.
[3]	Song Y,Feng L,Alyafei M A M,et al.Function of chloroplasts in plant stress responses[J].International Journal of Molecular Sciences,2021,22(24):13464.
[4]	Brocard C,Hartig A.Peroxisome targeting signal 1:is it really a simple tripeptide?[J].Biochim Biophys Acta,2006,1763(12):1565-1573.
[5]	Young J C,Barral J M,Ulrich Hartl F.More than folding:localized functions of cytosolic chaperones[J].Trends Biochem Sci,2003,28(10):541-547.
[6]	Baker M J,Frazier A E,Gulbis J M,et al.Mitochondrial protein-import machinery:correlating structure with function[J].Trends Cell Biol,2007,17(9):456-464.
[7]	Whitfield C,Mainprize I L.TPR motifs:hallmarks of a new polysaccharide export scaffold[J].Structure,2010,18(2):151-153.
[8]	Boudreau E,Nickelsen J,Lemaire S D,et al.The Nac2 gene of Chlamydomonas encodes a chloroplast TPR-like protein involved in psbD mRNA stability[J].Embo J,2000,19(13):3366-3376.
[9]	Sohrt K,Soll J.Toc64,a new component of the protein translocon of chloroplasts[J].J Cell Biol,2000,148(6):1213-1221.
[10]	Vaistij F E,Goldschmidt-Clermont M,Wostrikoff K,et al.Stability determinants in the chloroplast psbB/T/H mRNAs of Chlamydomonas reinhardtii[J].Plant J,2000,21(5):469-482.
[11]	Zhang Y,Peng Y,Liu J,et al.Tetratricopeptide repeat protein SlREC2 positively regulates cold tolerance in tomato[J].Plant Physiol,2023,192(1):648-665.
[12]	Kalanon M,Mcfadden G I.The chloroplast protein translocation complexes of Chlamydomonas reinhardtii:a bioinformatic comparison of Toc and Tic components in plants,green algae and red algae[J].Genetics,2008,179(1):95-112.
[13]	Livak K J,Schmittgen T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT Method[J].Methods,2001,25(4):402-408.
[14]	Cerveny L,Straskova A,Dankova V,et al.Tetratricopeptide repeat motifs in the world of bacterial pathogens:role in virulence mechanisms[J].Infect Immun,2013,81(3):629-635.
[15]	Lin Z F,Ho C W,Grierson D.AtTRP1 encodes a novel TPR protein that interacts with the ethylene receptor ERS1 and modulates development in Arabidopsis[J].J Exp Bot,2009,60(13):3697-3714.
[16]	Hu Z,Xu F,Guan L,et al.The tetratricopeptide repeat-containing protein slow green1 is required for chloroplast development in Arabidopsis[J].J Exp Bot,2014,65(4):1111-1123.
[17]	Schottkowski M,Ratke J,Oster U,et al.Pitt,a novel tetratricopeptide repeat protein involved in light-dependent chlorophyll biosynthesis and thylakoid membrane biogenesis in Synechocystis sp.PCC 6803[J].Mol Plant,2009,2(6):1289-1297.
[18]	Park S,Khamai P,Garcia-Cerdan J G,et al.REP27,a tetratricopeptide repeat nuclear-encoded and chloroplast-localized protein,functions in D1/32-kD reaction center protein turnover and photosystem Ⅱ repair from photodamage[J].Plant Physiol,2007,143(4):1547-1560.
[19]	Heinnickel M,Kim R G,Wittkopp T M,et al.Tetratricopeptide repeat protein protects photosystem Ⅰ from oxidative disruption during assembly [J].Proc Natl Acad Sci U S A,2016,113(10):2774-2779.
[20]	Larkin R M,Stefano G,Ruckle M E,et al.REDUCED CHLOROPLAST COVERAGE genes from Arabidopsis thaliana help to establish the size of the chloroplast compartment[J].Proc Natl Acad Sci U S A,2016,113(8):E1116-1125.
[21]	Rosado A,Schapire A L,Bressan R A,et al.The Arabidopsis tetratricopeptide repeat-containing protein TTL1 is required for osmotic stress responses and abscisic acid sensitivity[J].Plant Physiol,2006,142(3):1113-1126.
[22]	Dolferus R,Jacobs M,Peacock W J,et al.Differential interactions of promoter elements in stress responses of the Arabidopsis Adh gene[J].Plant Physiology,1994,105(4):1075-1087.
[23]	Signorelli S,Corpas F J,Borsani O,et al.Water stress induces a differential and spatially distributed nitro-oxidative stress response in roots and leaves of Lotus japonicus[J].Plant Science,2013,201-202(1):137-146.
[24]	Senthil-Kumar M,Hema R,Suryachandra T R,et al.Functional characterization of three water deficit stress-induced genes in tobacco and Arabidopsis:an approach based on gene down regulation[J].Plant Physiology and Biochemistry,2010,48(1):35-44.
[25]	梁燕,严建萍,谭湘陵.盐胁迫对水稻幼根乙醇脱氢酶和乙醛脱氢酶基因表达的影响[J].湖北农业科学,2012,51(13):2870-2872.
[26]	Katsuno T,Umeda K,Matsui T,et al.Deficiency of zonula occludens-1 causes embryonic lethal phenotype associated with defected yolk sac angiogenesis and apoptosis of embryonic cells[J].Molecular Biology of the Cell,2008,19(6):2465-2475.