Study on in Vitro Flowering Technology of Cymbidium Plant Cymbidium nanulum

FU Shuang-bin; YANG Yan-ping; YING Zhen; GAO Xu-yong; ZHOU Zhuang

doi:10.16590/j.cnki.1001-4705.2024.07.136

Seed > 2024 > 43(7): 136-142,149. > DOI: 10.16590/j.cnki.1001-4705.2024.07.136

FU Shuang-bin, YANG Yan-ping, YING Zhen, GAO Xu-yong, ZHOU Zhuang. Study on in Vitro Flowering Technology of Cymbidium Plant Cymbidium nanulum[J]. Seed, 2024, 43(7): 136-142,149. DOI: 10.16590/j.cnki.1001-4705.2024.07.136

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Study on in Vitro Flowering Technology of Cymbidium Plant Cymbidium nanulum

Zhejiang Academy of Agricultural Sciences, Zhejiang Institute of Subtropical Crops

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Received Date: February 23, 2024

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Abstract

Abstract

In order to protect, develop and utilize the new Cymbidium plant Cymbidium nanulum and accelerate the breeding process, this study induced in vitro flowering by direct rhizomes and indirect regenerated plants. The results showed that the highest number of normal flowers were obtained by direct induction on the adjusted MS medium containing 1 mg/L 6-BA, 30 g/L sucrose and 2 g/L peptone（165 mg/L NH₄NO₃, 380 mg/L KNO₃ and 850 mg/L KH₂PO₄）, with an average of 2.6. MS or 1/2 MS medium containing 3 mg/L 6-BA, 1 mg/L NAA and 100 mL/L coconut water was the most suitable medium for regeneration of Cymbidium nanulum rhizomes. In MS medium containing 3 mg/L 6-BA and 20 g/L sucrose, the number of flowers obtained by indirect induction was the highest, with an average of 0.8.
- orchid,
- Cymbidium nanulum,
- rhizomes,
- regenerated seedings,
- in vitro flowering

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References (39)

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