Abstract: Korean pine is an important timber tree species and nut economic forest tree species. The method of tissue culture was used, the immature seeds of five families from Sanchazi Korean pine seed orchard were selected as explants by tissue culture to study the embryogenic callus induction and cryopreservation. The results showed that immature seeds of different families had different induction rates of embryogenic callus on the medium supplemented with naphthaleneacetic acid（NAA） 2 mg/L + 6-benzylaminopurine（6-BA） 1 mg/L, and the best induction percentage was 5.68 %. The optimal sucrose concentration for the callus induction of Korean pine was 30 g/L. Embryogenic callus with good growth was selected for cryopreservation. After 10 days of recovery culture, it was observed that there were obvious new callus in different cell lines. After 15 days, a large number of callus proliferated. This study laid a foundation for large-scale propagation and conservation of fine germplasm resources of Pinus koraiensis in Sanchazi seed orchard.