Abstract: The penalty function model calculates the turning time from the beginning to the end of the turn. As a part of the delay impedance of the intersection, its size directly affects the calculation structure of the traffic impedance and traffic assignment. In order to reduce the error of delay model in simulation, the penalty function of freight car is recalibrated based on the actual data, and the penalty function of freight car in different speed range at intersection is obtained. Taking the intersection of Ningzhen Highway-Huanshan Road and Jiangdongbei Road-Dinghuaimen Street as examples, the penalty functions of minivan and heavy truck are calibrated and applied to simulation. The simulation results show that the new calibration of freight car penalty function has good fitting, and it is more accurate to describe the time of different vehicles passing through the intersection.