Abstract: To explore the differences and change rules of soil nutrients and enzyme activities in Larix gmelinii forest in the cold temperate zone, so as to provide scientific basis and data basis for in-depth understanding of forest ecosystem functions and future development trends in the cold temperate forest ecotone in China. In this paper, four different types of larch forest（Betula platyphylla-Larix gmelinii, Rhododendron dauricum-Larix gmelinii, Larix gmelinii, and Larix gmelinii plantation） in Heilongjiang Zhongyangzhan Black-Billed Capercaillie Nature Reserve were selected as the research objects. Sampling was carried out by season, and the soil. chemical properties and enzyme activities（catalase, acid phosphatase, urease, sucrase and cellulase） were determined in different seasons, and the dynamic changes with seasons were also explored. Results showed that:（1）Soil chemical properties and enzyme activities were significantly different in different forest types and seasons, and the changing trends were also different to some extent.（2）Soil acid phosphatase was significantly affected by the contents of soil total P, total N and available K. Soil catalase was significantly positively correlated with the contents of total N, available N, available K and available P. Soil sucrase was mainly affected by the contents of available P and available N. Soil urease and cellulase were most affected by soil organic carbon content.（3）The principal component analysis showed that there were significant differences in soil enzyme activities and soil chemical properties among the four forest types, and first principal component 1 could explain more than 50% of the variance variables. The soil chemical properties and enzyme activities in different Larix gmelinii were significantly different with the seasons, which were related to the accumulation of litter and the difference of vegetation types. Soil enzyme activities were correlated with soil chemical properties to varying degrees.