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松嫩平原盐碱地4种植物的光合荧光特性

Photochemical Fluorescence Properties of Four Plants in Saline-Alkaline Soil of Songnen Plain

  • 摘要: 以吉林省西部轻度盐碱地种植的沙枣、枸杞、蒙桑和柠条锦鸡儿实生苗为试验材料,针对4种植物的光合参数和叶绿素荧光参数等相关方面进行差异性和相关性分析,揭示4种植物的光合性能,以期为盐碱地治理提供参考。结果表明,4种植物的光合特征和叶绿素荧光特性差异显著,净光合速率(P_n)由大到小依次为:枸杞、蒙桑、沙枣、柠条。试验植物叶片的净光合速率(P_n)与气孔导度(Gs)有显著的正相关性,因而P_n受Gs影响较大;并且P_n与光适应下的最大光化学效率、实际光化学效率(ФPSⅡ)、光化学淬灭系数(qP)和电子传递效率(ETR)等荧光参数密切相关。枸杞的J相和I相的相对可变荧光速率(Vj和Vi)最低,质体醌电子传递效率高,光系统(PSⅡ)受体侧的质体醌库(Sm)最大,光反应活性最大。柠条电子传递效率低,光反应活性受到天线色素对能量(ABS)的吸收和反应中心能量捕获(TR)的限制。同时,枸杞的性能指数(PI ABS)在以初始荧光(F_o)为基础的性能指数(PI CS_o)和以最大荧光(Fm)为基础的性能指数(PI CSm)显著高于其他树种,柠条最低。4种植物的3个性能指数之间具有显著差异性和极显著相关性,且P_n与PI ABS、PI CS_o、PI CSm呈显著相关性。因此,PI ABS、PI CS_o和PI CSm适合作为4种植物叶绿素荧光特性的评价指标。研究表明,4种植物中枸杞的性能指标(PI)和P_n最高,因而光合性能最强,蒙桑和沙枣次之,柠条最低。所以枸杞的光合生理活性强,适合本试验盐碱地环境的栽植和引种。

     

    Abstract: Using the seedlings of Elaeagnus angustifolia; Lycium barbarum; Morus mongolica and Caragana korshinskii planted in mild saline-alkali land in the western part of Jilin Province as test materials, the differences and correlations of photosynthetic and chlorophyll fluorescence parameters of the four plants were studied, in order to reveal the photosynthetic performance of the four plants and provide a reference for the application of saline-alkali land greening plants. The results showed that there were significant differences in photosynthesis and chlorophyll fluorescence of the four plants. The net photosynthetic rate(Pn) was in the order of Lycium barbarum,Morus mongolica, Elaeagnus angustifolia and Caragana korshinskii. There was a significant positive correlation between net photosynthetic rate(Pn) and stomatal conductance(Gs), so Pn was greatly affected by Gs, and Pn was closely related to fluorescence parameters such as maximum quantum yield of primary PSⅡ photochemistry under light adaptation; actual quantum yield of primary PSⅡ photochemistry(ФPSⅡ); photochemical quenching(qP); electron transfer efficiency(ETR) and so on. The relative variable fluorescence Vj and Vi of J phase and I phase of Lycium barbarum were the lowest, the electron transfer efficiency of plastoquinone was high, the plastid plastoquinone(Sm) on the receptor side of PSⅡ was the highest, and the photosystem activity was the highest. The electron transfer efficiency of Caragana korshinskii was low, and the photoreaction activity was limited by the energy absorption of antenna pigment(ABS) and the energy capture of reaction center(TR). At the same time, the performance index(PI ABS) of Lycium barbarum was significantly higher than that of other tree species in the performance index(PI CSo) based on initial fluorescence(Fo)and the performance index(PI CSm) based on maximum fluorescence(Fm), and Caragana korshinskii was the lowest. There were significant differences and extremely significant correlation among the three performance indexes of the four plants, and there was a significant correlation between Pn and PI ABS, PI CSo, PI CSm. Therefore, PI ABS, PI CSo, PI CSm was suitable to be used as an evaluation index of chlorophyll fluorescence characteristics of four kinds of plants. According to research, among the four plants, Lycium barbarum had the highest performance index PI and Pn, and therefore had the strongest photosynthetic performance, followed by Morus mongolica and Elaeagnus angustifolia, and Caragana korshinskii was the lowest. Therefore, Lycium barbarum had strong photosynthetic physiological activity and was suitable for planting and introduction in the saline-alkali environment of this experiment.

     

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