Abstract: The study was conducted to develop an efficient high performance liquid chromatography（HPLC） method for simultaneous determination of seven taxanes in Taxus, the contents of seven taxanes in three Taxus species were compared and analyzed. HPLC detection conditions were: the chromatographic column was HiQsil C₁₈ column; the binary mobile phases was acetonitrile（A）-water（B）; the gradient elution procedure was（0～10 min,30%A;10～12 min,30% ～ 45%A;12～ 25 min,45%A;25～ 30 min,42% ～ 45%A;30～40 min,30% ～42%A）; the detection wavelength was 227 nm. Linear regression was carried out for the mass concentration and peak area of each taxane component, and the sample recovery of 7 taxanes at different addition levels was investigated. The results showed that the contents of paclitaxel in the bark of Taxus cuspidata, Taxus chinensis var. mairei and Taxus×media cv. hicksii were 0. 234, 0. 043, 0. 167 mg/g, the contents of Taxol B were 0. 092, 0. 034, 0. 053 mg/g, the contents of 10-DAB were 0. 376, 0. 158,0. 063 mg/g, the contents of 10-DAT were 0. 157, 0. 113, 0. 126 mg/g, the contents of Baccatin Ⅲ were 0. 395, 0. 116, 0. 074 mg/g, the contents of 7-epi-10-DAT were 0. 053, 0. 027, 0. 033 mg/g, the contents of 7-xyl-10-DAT were 0. 266, 0. 564, 0. 315 mg/g. Taxanes in Taxus cuspidata were the highest, followed by Taxus cuspidate, and the lowest in Taxus×media cv. hicksii. The seven taxane compounds had good linear relationship within the linear range determined, and the test results of sample addition recovery rate showed that the recovery rate of this method was high. The established HPLC analysis method can simultaneously determine the contents of seven taxanes in Taxus, and this study provides a basis for the quality control, provenance breeding and resource development of Taxus.