Abstract: The effects of exogenous auxin IBA on cutting rooting of T.amurensis was studied in the greenhouse, the related physical and chemical characteristics in the rooting process were analyzed to provide support and theoretical guidance for the cutting propagation technology of T.amurensis. Different concentrations of IBA were used to treat the semi-lignified cuttings of T.amurensis, the rooting situation was recorded. The samples were collected regularly to detect the effect of IBA on oxidase activity, endogenous hormone content of cuttings. Results showed that the maximum rooting rate, average root length and root fresh weight of T.amurensis were obtained under 100 mg/L IBA with treatment time 8 h, which were 89.52%, 14.19 cm and 913.43 mg, respectively, better than other treatments. In the rooting process of IBA treatment, the activities of SOD and POD showed a low level at 20 d and 40 d, and a peak value at 30 d. The activities of PPO were lower at 20 d, 30 d and 40 d. The activities of IAAO showed a low level at 20 d and 30 d, and a peak value at 40 d. The content of IAA, the IAA/ABA value showed a low level at 20 d and 40 d, and a peak value at 30 d. The content of GA₃ showed a valley value at 20 d, then increased and maintained a high level. The content of ABA maintained at a low level for 0-30 d, and then increased. The content of ZR showed two peaks at 20 d and 40 d, and a valley value at 30 d. From this, it can be concluded that 100 mg/L IBA with treatment time 8 h had the best rooting effect. The low activity of SOD, POD, PPO and IAAO was beneficial to the formation of callus at the early stage of cutting（20 d）, and the high activity of SOD and POD was beneficial to the formation of adventitious roots（30 d）, and the high activity of IAAO was beneficial to the elongation of adventitious roots（40 d）. Higher ZR content was required in callus formation（20 d）, and higher IAA, GA₃ content were required in adventitious root formation（30 d）, and higher GA₃, ABA, ZR content were required in adventitious root elongation and development（40 d）. The lower IAA/ABA and IAA/ZR was beneficial to callus formation and adventitious root elongation, while the higher was beneficial to adventitious root formation.