Abstract: In order to understand the effects of different fertilization treatments on endogenous hormones and growth of Juglans mandshurica Maxim, a three-factor orthogonal fertilization test of N, P and K was conducted to determine the endogenous hormone contents and growth traits of 6-year-old Juglans mandshurica Maxim clones as test materials. The results showed that fertilization helped to increase the contents of four endogenous hormones of Juglans mandshurica Maxim, namely trans-zeatin（tZ）, gibberellin（GA₃）, abscisic acid（ABA） and indole acetic acid（IAA）, among which the content of trans-zeatin（tZ） changed the most; the three kinds of fertilizers, namely, nitrogen, phosphorus and potassium, had different influences on the endogenous hormones, and the influences on the contents of trans-zeatin（tZ） and gibberellin（GA3） were N＞K＞P, and the role of affecting abscisic acid（ABA） and indole acetic acid（IAA） content was N＞P＞K; nitrogen fertilizer was the optimal factor to enhance the content of the four endogenous hormones, and the combination of fertilization treatments to enhance the content of the four endogenous hormones in the comprehensive analysis was 0.75 kg/plant of nitrogen fertilizer, 0.3 kg/plant of phosphorus fertilizer, 0.15 kg/plant of potash fertilizer. In terms of growth, fertilization reduced the variation in tree height within the asexual seed source, but the difference in net growth in tree height between the different fertilization treatments was not significant, and the different fertilization treatments did not have a significant effect on the promotion of new shoot length and coarseness.