松嫩平原植被演替对土壤微生物的影响
Effects of Vegetation Succession on Soil Microorganisms in Songnen Plain
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摘要: 为探寻盐碱地土壤微生物功能多样性随植被演替的变化规律,采用Biolog Eco微平板法,对松嫩平原盐碱地自然演替序列和人工恢复后不同植被土壤微生物群落功能多样性进行研究。结果表明,自然演替序列下,随着植被从碱斑光板地演替为羊草顶级群落,土壤微生物碳源代谢水平显著增加(P<0.05),96 h每孔平均吸光度(Average Well Color Development,AWCD)从0.04增至0.61, Shannon多样性指数(H)从1.43增至3.20,Simpson优势度指数(D)从0.70增至0.96,McIntosh指数(U)从0.75增至4.03。人工恢复后,草本及灌草植物群落土壤微生物利用碳源数量最多,人工林植物群落利用碳源数量降低。不同植物群落下土壤微生物AWCD、Shannon多样性和丰富度指数、Simpson优势度指数及McIntosh指数与土壤有机质、全氮含量呈极显著正相关(P<0.01),与土壤密度、pH和总盐含量呈极显著负相关(P<0.01)。Abstract: In order to explore the changes of soil microbial functional diversity in saline alkali land with vegetation succession,Biolog Eco microplate method was used to study the natural succession sequence and the functional diversity of soil microbial community of different vegetation after artificial restoration in Songnen Plain saline-alkali land. The results showed that the carbon source metabolism of soil microorganisms increased significantly(P<0. 05), the Average Well Color Development(AWCD) increased from 0. 04 to 0. 61 at 96 h, Shannon diversity index(H) increased from 1. 43 to 3. 20, Simpson dominance index(D) increased from 0. 70 to 0.96, and McIntosh index(U) increased from 0. 75 to 4. 03 with the succession of vegetation from alkali spot to leymus chinensis. After artificial restoration, the amount of carbon source used by soil microorganisms in herbaceous and shrub-grass communities was the largest, while the amount of carbon source used by soil microorganisms in artificial forest communities decreased. Soil microbial AWCD,Shannon diversity and richness index, Simpson dominance index and McIntosh index were significantly positively correlated with soil organic matter and total nitrogen content(P<0. 01), and significantly negatively correlated with soil density, pH and total salt content(P<0. 01).