Abstract: In order to increase the types of color-leaf tree species in the cold northern regions, the greening company in the cold northern regions established a rapid propagation system of cold-tolerant American red maple, and increased the types of color-leaf tree species in the north, especially in the northeast. In this experiment, the ‘Autumn Blaze’ of Acer rubrum L was taken as the research object, and the axillary bud stem segment was used as the explant in vitro culture. The medium formula for the sterilization of the explant, start culture, subgeneration culture, strong seedling culture, and rooting culture was carried out by orthogonal experiment and two-way analysis of variance. The rapid propagation system was established through four stages of primary cultivation, secondary propagation cultivation, strong seedling cultivation, and rooting cultivation. The results showed that the disinfection combination was 5% NaClO+0. 1%HgCl₂, the average pollution rate was 33. 15%, the average survival rate was 60. 98%, and the mortality rate was 5. 87%. The best proliferation medium was modified MS+0. 4 mg/L CPPU+0. 1 mg/L IBA+1. 0 mg/L 6-BA, the multiplication coefficient was 6. 45. The best seedling medium was modified MS+1. 0 mg/L ZT+0. 5 mg/L IBA, the average plant height was 6. 15 cm, and the stem thickness was 21. 41 mm. The best rooting induction medium was 1/2 MS+0. 1 mg/L NAA+0. 1 mg/L IBA, and the rooting rate was 100%. This rapid propagation system is highly efficient and suitable for production.