Abstract: In view of insufficient high-temperature performance and elastic recoverability of the existing plant oil regenerated bitumen, in this study, rock bitumen（RB） and plant oil（PO） were utilized to regenerate the aged bitumen. The variation law on the physical properties of regenerated bitumen binder was analyzed through conventional three indexes test and Brookfield viscosity（RV） test, and the comparative evaluation on high-and low-temperature rheological characteristics and fatigue behavior of regenerated bitumen binder was implemented via frequency sweep（FrS）, temperature sweep（TeS）, multiple stress creep recovery（MSCR）, bending beam rheological test（BBR） and linear amplitude sweep（LAS） test. The results demonstrated that: 10% PO content could restore the corresponding physical properties to the level before aging, except for insufficient high temperature properties. The combination of RB and PO could significantly improve the high temperature elastic deformation and recoverability of plant oil regenerated bitumen, and increase the critical temperature T_c value; meanwhile, it could increase the yield strain and prolong fatigue life; furthermore, the combination of RB and PO might weaken the low-temperature creep properties to a certain extent with regarding to regenerated bitumen but the effect was relatively low. After comprehensive consideration of high and low temperature performance and fatigue properties, the utilization of 10%PO and 6%RB could achieve the optimum composite regeneration effect on the aged bitumen.