Abstract:
This experiment used an indoor culture dish germination method to study the effects of exogenous addition of polyethylene glycol, sodium carbonate, and sodium bicarbonate on the embryos germination and physiological indicators of S. pohuashanensis seedlings. The results showed that polyethylene glycol treatment increased the germination percentage of S. pohuashanensis embryos by 15%to 25%, while sodium carbonate and sodium bicarbonate treatment reduced the germination percentage of S. pohuashanensis embryos by 88% and 85%, respectively. Polyethylene glycol can alleviate the inhibition of saline alkali stress on the germination of S. pohuashanensis embryos, and increase the germination rate of embryos under stress by 1-2 times. Polyethylene glycol can increase the activity of antioxidant enzymes in the embryos of S. pohuashanensis under saline alkali stress, resulting in an increase of 16. 45%, 75%, and 80% in enzyme activities such as SOD, POD, and CAT, respectively. Polyethylene glycol reduced MDA in embryos under stress by 41. 44%. Research has shown that polyethylene glycol can effectively alleviate the inhibitory effect of salt alkali stress on the embryos of S. pohuashanensis, improve the activity of antioxidant enzymes in S. pohuashanensis embryos, enhance the stress resistance of S. pohuashanensis, and provide scientific basis for establishing PEG priming technology to promote the germination of S. pohuashanensis embryos.