Abstract: In order to optimize the technical problems of cutting rooting of rare and endangered tree species Euptelea pleiospermum, using 1-year-old semi lignified branches of E. pleiospermum wood as cutting materials, an orthogonal experimental design was adopted to explore the effects of different substrates, exogenous hormones, concentrations, and treatment times on the rooting of E. pleiospermum wood cutting through the calculation of rooting rate, root effect index, and other indicators（Matrix type、hormone type, mass concentration, hormone treatment time were four factors, and each factor had four gradients）. The membership function numerical method was used to comprehensively evaluate the rooting effect of 16 treatment combinations, providing technical reference for the expansion of E. pleiospermum wood. Among the four cutting substrate ratios（100% peat;V（peat）:V（perlite）=5:2; V（peat）:V（vermiculite）=5:2; V（peat）:V（perlite）:V（vermiculite）=5:2:2, V represents the volume ratio）, peat: perlite（5:2） had the best rooting effect, with a rooting rate, average root length, longest root length, average root number, and average root diameter of 56. 67%, 6. 74 cm, 7. 47 cm, 5. 73 stripes/ears, and 0. 31 cm, respectively. The root effect index reached 1. 74, which was much higher than the other three substrate ratios（P＜0. 05）. The results of the orthogonal experiment showed that the rooting effect of cuttings soaked with ABT-1 at a concentration of 1 g/L for 5 seconds was the best, with a rooting rate of 65. 73%, an average root length of 7. 38 cm, an average number of 7. 59 stripes/ears, a maximum root length of 7. 52 cm, an average root diameter of 0. 35 cm, and a root effect index of 1. 87. Comprehensive analysis showed that the optimal treatment combination for rooting effect was（peat to perlite volume ratio 5:2 as substrate, with the highest average membership function value in ABT-1 at a medium speed of 1. 0 g/L for 5 seconds）, and the lowest treatment combination was(peat to vermiculite volume ratio 5:2 as substrate, soaked in 0. 8 g/L indole butyric acid（IBA） for 12 hours.