Abstract: In order to improve driving safety and comfort of drivers on curved roads in forest areas, a driving simulation test was adopted to evaluate the driving comfort on curved roads in forest areas from a dynamic perspective using eye movement and heart rate as evaluation indexes. Based on the theory of spatial sight distance, five different spatial visual distance scenarios of curved roads in forest areas were established, and the results showed that the rate of change of pupil diameter and the growth rate of heart rate of the driver decreased with the increase of spatial sight distance, and the driving comfort increased continuously. The minimum spatial sight distance values considering the pupil diameter change rate and heart rate growth rate were 101 m and 89 m, and the appropriate spatial sight distance values considering the pupil diameter change rate and heart rate growth rate were 128 m and 132 m.