Effects of IAA, NAA and IBA on Rooting of Tissue Culture Seedlings of Cranberry
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Abstract
Cranberry(Oxycoccos) has strong cold resistance, and its fruit has high health value and economic value. In this study, the tissue cultured seedlings of cranberry were used as cuttings to explore the soaking and quick dipping tests of cranberry cuttings under different indole-3-acetic acid(IAA), 1-naphthlcetic acid(NAA) and 3-indolebutyric acid(IBA) concentrations, and the effects of different hormone types and concentrations on rooting rate, seedling height growth, biomass and root growth characteristics(including the longest root length, root surface area, average root diameter and average root number). The results showed that, when NAA soaking treatment was 100 and 200 mg/L, the root system grew well, but the longest root length was only(30.77±0.18) cm(NAA 100 mg/L), and the average root diameter was(0.3±0.02) mm. However, the longest root length of cuttings treated with IBA 1000 mg/L per hour was(30.13±3.22) cm, the average root diameter was(0.39±0.04) mm, the root surface area was(1.27±0.01) cm~2, and the average number of roots was(128.22±2.38). From this point of view, there was little difference in root growth between the soaking treatment and the rapid soaking treatment, but in general, when the IBA concentration was 1000 mg/L, the rooting rate, was 85% at the highest. Therefore, the quick dipping method was more suitable for the rooting of cranberry tissue culture seedlings. In this study, the cutting propagation technology of tissue culture seedlings of cranberry was established for the first time, which laid the foundation for large-scale propagation of cranberry.
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