Abstract: Based on the background of national ecological civilization construction in the new period, such as the great protection of the Yangtze River and the ecological economic belt of the Han River, in order to clarify the current ecological water demand of Yue River at the basin scale, the main tributary of Han River, and to propose the targeted ecological water refill scheme to recover the water ecological environment of the Yue River. Taking the main stream of Yue River as the research object, the research flowchart of eco-combined was proposed in basin scale, and the eco-combined model of reservoir group considering the ecological environment objective of different “good” or “very good” grades was established and solved. The results are as follows. It was difficult to meet the high ecological demand of the main stream of Yue River only by discharging the established ecological base flow from the main reservoir in the basin. The quantity of ecological environment water increased significantly under the eco-combined operation, which can meet the standard of “good” ecological environment of the main stream of the Yue River. In terms of temporal and spatial pattern on the ecological water shortage, the middle of the river is the key area, and the period from January to March is the key period. The research results are expected to improve the ecological environment quality of the Yue River trunk stream, optimizing the water resources allocation, and supporting the high-quality development of economy and society in the Yue River basin.