Abstract: To analyze the dynamic response of large-span prestressed beamless floor of slab-column-wall structure under vertical earthquake, a simplified model of three-story cold store in area with earthquake intensity of seven was taken as an example. Firstly, structural design was conducted using YJK software, and the static loading and dynamic time history were then analyzed by ABAQUS software. The dynamic loading condition includes three-way ground motion of small earthquake, horizontal bidirectional ground motion of strong earthquake, vertical ground motion of strong earthquake, as well as three-way ground motion of strong earthquake. The structural plastic damage, vertical acceleration distribution of each floor, column axial force of each floor, punching force of slab-column connections and vertical earthquake action were analyzed, and the rules of vertical earthquake influence coefficient and dynamic coefficient were summarized. The results are as follows. Vertical earthquake can increase the damage degree of slab-column connections. The vertical acceleration of large-span prestressed beamless floor at each point under vertical earthquake is not uniform. The column axial force and punching force of slab-column connections increase significantly under three-way strong earthquake loading condition. The maximum value of seismic influence coefficient of structure under vertical strong earthquake conditions is 0.556. The rule of dynamic coefficient varying with height is related to the characteristics of seismic wave spectrum, and the maximum value of 3.89 was obtained. It is suggested that measures should be taken to enhance the bearing capacity and seismic performance of key structure component according to the analysis results in practical engineering design.