Abstract: A simple, rapid and accurate method for the determination of monosaccharide compositions from lignocellulose byionchromatographywas established. The HPAEC-PAD method was established to simultaneously detect 7 different monosaccharides and two types of alduronic acid. Dionex ICS-5000⁺ with CarboPAC^TMPA10(4 mm×250 mm) column and pulsed amperometric detection was involved in this method. This method could be simultaneously qualitatively and quantitatively analysed by the gradient elution of NaOH and CH₃COONa.The results showed that the method has good linearity (R²≥0.999 0), high precision (RSD < 3%), adequate (98.53%-118.63% recovery) and good reproducibility (RSD < 1.16%)within the range. The limitions of quantitation and detection were 0.05-3.0 mg/L and 0.01-1 mg/L, respectively. The method was applied to determine the monosaccharide compositions from bamboo leaves hydrolysate.The results showed that bamboo leaves hydrolysate was composed of arabinose, galactose, glucose and xylose.The mole ratio of monosaccharide to n(arabinose) : n(galactose) : n(glucose) : n(xylose)=2.9 : 0.7 : 8.2 : 9.7.This method is successful and can be used for the determination of monosaccharides in lignocellulose.