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茶皂素提取条件的优化及纯化研究

Study on Extraction and Purification of Tea Saponin from Tea Seed Meal

  • 摘要: 以油茶茶籽粕为原料,采用乙醇水溶液提取茶皂素。在茶籽粉和乙醇料液比1 : 9(g : mL),乙醇体积分数60%,提取温度60 ℃和提取时间3 h的最佳条件下茶皂素的提取得率达14.9%。用NKA-9型大孔吸附树脂吸附纯化茶皂素粗品,树脂静态吸附与解吸结果表明:树脂静态吸附茶皂素粗提液0.5 h基本饱和,体积分数80%乙醇解吸率为91.1%;动态吸附与解吸时,上样流速8 mL/min较佳,吸附率为66.04%,体积分数80%乙醇洗脱,洗脱流速5.0 mL/min,洗脱体积50 mL时,可使流出液中茶皂素质量浓度在1.25~1.57 g/L之间,茶皂素纯度为95%。

     

    Abstract: Tea saponin was extracted by alcohol extraction using tea seed meal as raw material. The optimum extraction conditions of tea saponin was as follows:the ratio of solid to liquid was 1 : 9(g/mL), the volume fraction of extraction solution(ethanol) was 60%, the extraction temperature was 60 ℃ and the extraction time was 3 h, under the above reaction conditions, the highest yield of tea saponin was 14.9%. Crude tea saponin was purified by NKA-9 macroporous resin. In the purification experiment, the static adsorption reached saturation for 0.5 h and the desorption rate of the volume fraction 80% ethanol reached the maximum of 91.1% in the static desorption, while the dynamic adsorption and desorption. The adsorption rate reached 66.04% at the rate of 8 mL/min, and the concentration of tea saponin was between 1.25 g/L and 1.57 g/L after elution with the volume fraction 80% ethanol of 50 mL at the flow rate of 5.0 mL/min, tea saponin purity 95%.

     

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