Abstract:
Tea saponin was extracted by alcohol extraction using tea seed meal as raw material. The optimum extraction conditions of tea saponin was as follows:the ratio of solid to liquid was 1 : 9(g/mL), the volume fraction of extraction solution(ethanol) was 60%, the extraction temperature was 60 ℃ and the extraction time was 3 h, under the above reaction conditions, the highest yield of tea saponin was 14.9%. Crude tea saponin was purified by NKA-9 macroporous resin. In the purification experiment, the static adsorption reached saturation for 0.5 h and the desorption rate of the volume fraction 80% ethanol reached the maximum of 91.1% in the static desorption, while the dynamic adsorption and desorption. The adsorption rate reached 66.04% at the rate of 8 mL/min, and the concentration of tea saponin was between 1.25 g/L and 1.57 g/L after elution with the volume fraction 80% ethanol of 50 mL at the flow rate of 5.0 mL/min, tea saponin purity 95%.