Abstract: Chitosan(CS)/polyvinyl alcohol(PVA)/chitosan oligosaccharide(CHOS) nanofibrous membrane was prepared by electrospinning. The morphology, structure, bacteriostasis, hydrophilicity and solubility of the membrane were studied. Scanning electron microscope(SEM) observation showed that CS/PVA/CHOS nanofibrous membrane had uniform and dense micro morphology. FT-IR test showed that CHOS was dispersed in CS/PVA/CHOS nanofibrous membrane in the form of physical mixing. XRD test showed that the addition of CHOS changed the crystallinity of the nanofibrous membrane and promoted the compatibility between components. Water contact angle test showed that the nanofibrous membrane had good hydrophilicity. As the mass ratio of CS, PVA and CHOS was 20:80:10, the water contact angle of CS/PVA/CHOS nanofibrous membrane decreased from 59.8° to 37.5° compared with that of CS/PVA nanofibrous membrane with the mass ratio of CS and PVA 20:80. Bacteriostatic test and solubility test showed that CS/PVA/CHOS nanofibrous membrane with mass ratio of CS, PVA and CHOS 20:80:10 were better than those of CS/PVA nanofibrous membrane without CHOS, the bacteriostasis and solubility increased 38.9% and 38.6%, respectively.