Abstract: In order to improve the tapping yield of Pinus yunnanensis oleoresin, a nutritional tapping irritant agent was used. The efficiency of the agent and the influence of it to the physiological indexes of P. yunnanensis needles were investigated. The results showed that the maximum tapping yield increase reached to 41.08% with an average increase rate of 34.76%. The wet weight, dry weight, total chlorophyll content and soluble protein content of P. yunnanensis needle increased by 11.02%, 10.26%, 13.03% and 40.96%, respectively. The contents of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) in the needle cells of P. yunnanensis also increased, which increased the resistance of P. yunnanensis. The content of malondialdehyde(MDA) and the relative electrical conductivity in pine needles decreased, which increased the stability of endomembrane system and enhanced the ability of P. yunnanensis to resist adversity.