Abstract: Five kinds of tara tannin with different contents were determined and compared by UV spectrophotometry and hide powder method, and the significance test of tannin content determination results was carried out by SPSS software. The results showed that there was no significant difference in the determination of tannin content of high-puritytaratannin(93%) measured by UV spectrophotometry and hide powder method(P>0.05).However, the experimental results of the four kinds of tara tannins with the content of 60% have significant differences(P < 0.05), and the tannin contents measured by hide powder method were all higher than those measured by UV spectrophotometry, where the difference was about 1.5%-2.0%. And the correction constant i.e., p=1.03, in the formula for determining the taratannin content by UV method was calculated. The results of UV-Vis absorption spectrum and high performance liquid chromatography analysis showed that the maximum absorption peaks of tara tannin and gallic tannin were both at 276 nm. At the same mass concentration, the absorption peak intensity of tara tannin was higher than that of gallic tannin. The peaking time of tara tannin was mainly in 20-40 min, and the peaking time of gallic tannin was mainly in 30-45 min.