Abstract:
The coupling system of hydrogen peroxide and attapulgite was used to decolorize
Camellia oleifera saponin. The effects of the amount of hydrogen peroxide, temperature, time, and attapulgite amount on the decolorization rate were investigated, and the optimal conditions for decolorization of saponins were explored. The advantages and disadvantages of different color evaluation methods, such as spectrophotometer, spectroradiometer, Lovibon colorimeter, color scale colorimeter, and visual photography, on the color determination of saponins were compared. The results showed that the optimum decolorization conditions were 6% H
2O
2, 80 ℃, 3 h, 3% attapulgite, and the milky white
Camellia oleifera saponin. The results of five color evaluation methods are basically similar. The color of
Camellia oleifera saponin could be accurately and quantitatively analyzed by spectrophotometer. The spectroradiometer method was suitable for color evaluation with small color difference. The Lovibond colorimeter method had advantages in the scene where a wide range of color analysis of
Camellia oleifera saponin solution was required. Color scale colorimetry has simple operation and high feasibility. Visual photography could support the above methods.