Abstract: In this study, for the preparation of biomass-based membrane materials with good mechanical properties and antimicrobial activity, bamboo pulp board was used to prepare dialdehyde cellulose(DAC), which contained 7.99 mmol/g aldehyde groups by sodium periodate oxidation and hot water extraction. Bamboo-based oxide fiber film materials(DAC-DGEG, DAC-G, DAC-GTE, DAC-SA) were prepared by solution casting method via adding cross-linking agents of poly diglycidyl ether of diethylene glycol(DGEG), glycerol(G), glycerin tri-glycidyl ether(GTE), and sodium alginate(SA) mixed at a mass ratio of 4∶1, respectively. Scanning electron microscopy(SEM), Fourier infrared spectroscopy(FT-IR), and mechanical testing were used to investigate the microstructure, chemical structure, mechanical properties and anti-bacterial activities of composite films. The results indicated that tensile strength and elongation at break of DAC film was 11.7 MPa and 0.46%, respectively. In the visible range, the transmittance of DAC film was 92%. Adding crosslinking agents into DAC enhanced mechanical property of composite films. Glycerol and sodium alginate could effectively improve mechanical properties of the DAC films. The corresponding composite membranes, DAC-G and DAC-SA, enhanced the tensile strength by 125% and 113%, and the elongation at break increased by 6.48 and 5.09 times, compared with those of DAC membranes. The effect of the addition of cross-linking agent on the optical properties and antimicrobial activity of the DAC membranes was not obvious, and the light transmittance of DAC-G and DAC-SA composite membranes was 90% and 88%. The aldehyde group in the composite membranes had good activity, and showed excellent inhibition of E. coli, S. aureus, C. albicans and B. subtilis.