Abstract: Extracts from Toddalia asiatica (Linn.) Lam were obtained under the room temperature and hot refluxing with water, absolute alcohol,ethyl acetate and petroleum ether. Antibacterial effects of extracts from T. asiatica on Bacillus subtilis, Shigella dysenteriae and Saccharomyces cerevisiae were compared. The results showed: There was no remarkably different antibacterial activity of extracts under the room temperature for 72 h and hot refluxing for 5 h to three bacteria (p>0.05). There was remarkably different antibacterial activity of extracts of absolute alcohol and ethyl acetate in comparison with water extract (ppT. asiatica, and the suitable pH values for inhibition of the three bacteria were different.