Abstract: The Jew's-ear protein extraction by ultrasonic method was studied. High power ultrasonic was used to disrupt cell to increase extraction yied of Jew's-ear protein. The main influence factors on protein yield were explored by single factor test and orthogonal test. The optimal extraction conditions were 25 min of irradiation time,1:50 of solid-liquid ratio,3 times of irradiation. The extraction yield of protein was 2.60%. Comparing with traditional technique, the extraction time decreased dramatically and extraction yield increased markedly.