Abstract: Reference materials of abietic acid were prepared using abietic acid with purity of 95% isolated from Pinus massoniana Lamb. rosin. Quantitative analysis of abietic acid was carried out by gas chromatography using DB-5 quartz capillary column. Homogeneity examination of the reference materials was applied by statistical analysis. The stability of the reference materials of abietic acid was good and amounted to 8 months at -16℃.