Abstract: The content of methyl gallate (MG) was determined by HPLC. The characteristic absorption of MG was determined by ultraviolet scanning and the wavelength of characteristic absorption was 279 nm. The results showed that relationship between peak area of MG and the content of sample solutions in definite range obey Lombard Beer's law. The measured values were analyzed by statistical method, and it obey the normal distribution. The standard deviation of measured values was 0.02% which was within the limit of requirements. Statistic analysis showed that the measured values from two laboratories using F test and t test had no significant difference, which demonstrated that the method for determination of MG had high precision and accuracy. The method appeared to be simple and quick, cheap and stable.