Abstract: The four types of essential oil of Eucalyptus leaves from Africa were obtained by steam distillation and analyzed by gas chromatography/mass spectrometry(GC-MS).The compounds of 1# and 2# oil were eucalyptol, terpinyl acetate, α-gurjunene, aromadendrene, alloaromadendrene, ledene, globulol and ledol. The compounds of 3# and 4# oil were p-cymene, eucalyptol, cryptone, cumaldehyde, phellandral, spathulenol, eudesmol, and 9,12-octadecadienoic acid.