Abstract: Based on the analysis of bio-oil properties, the current status of bio-oil separation and purification technology is summarized, including distillation, fractional condensation, solvent separation, centrifugal separation, chromatography, membrane separation and supercritical extraction. The main problems existing in bio-oil separation are summarized. Investigation trend of the technology is presented. It is suggested that energy utilization and chemical product separation can be integrated through fractional condensation and economical benefits can be maximized. It is also suggested that membrane separation and supercritical extraction have potential research value in the separation of bio-oil.