Abstract: Preparation of biodiesel as well as recovery and refining of glycerol from the by-product of biodiesel was researched.The effects of solvent,pH value of solution and distilling temperature on yield of pure glycerol are also discussed.The optimal process conditions for the transesterification(reaction) of rapeseeds oil are:methanol to oil ratio at 6 :1,1% KOH(W%) of oil,reaction temperature 60℃,reaction time 90 min.Fatty acid methyl ester yield is more than 98.1% at the best technology conditions.The best operations for the recovery and refining technology of glycerol are as follows: the bottom layer of transesterification product was diluted with methanol,then was neutralized to pH=5.0-7.0.The purity of refined glycerol is 97.52%.