提取方法对茶油中活性成分角鲨烯含量的影响

Influence of Extraction Method on Content of Bioactive Component Squalene in Seed Oil of Camellia Oleifera Abel

摘要: 采用不同的工艺和溶剂从油茶籽中提取茶油,并对经甲酯化处理的茶油进行气相色谱分析、GC-MS联用分析、标准物质共同色谱比对等方法,研究了不同的提取方法对茶油中生物活性物质角鲨烯含量的影响。结果表明,茶油中含有比较多的角鲨烯,其含量随提取方法而不同,直接压榨法、己烷提取法、石油醚提取法、丙酮提取法和三氯甲烷提取法等所得到的茶油中角鲨烯的含量分别为2.98%、7.62%、0.94%、0.29%和0.54%。

Abstract: The bioactive compound squalene was firstly detected in seed oil of Camellia oleifera Abel by GC analysis,GC-MS(analysis) of the methyl ester of the seed oil,and co-chromatography with standard compounds.Influence of extraction method on squalene content in the seed oil was studied.The research results show that squalene in the seed oil is on relative high level,and the squalene content changes with the difference of extraction method or solvents.The squalene content in the seed oil from direct expression method,extraction with hexane,petroleum ether,acetone,and chloroform is 2.98%,7.62%,0.94%,(0.29%),and 0.54%,respectively.