茄秆中生物总碱提取条件的研究

Study on Extraction of Alkaloids from the Stem of Solanum melongena Linn.

摘要: 以茄秆为原料,用索氏提取法进行生物总碱的提取,用酸碱滴定法对提取的生物总碱的含量进行测定。以生物总碱的含量作为指标,进行单因素试验,研究了有机溶剂种类、料液比、提取时间、pH值等因素对茄秆中生物总碱提取的影响。茄秆生物总碱的优化条件为: 60% 乙醇为提取剂,料液比为1:5.5, pH值为3,时间为 3 h,生物总碱平均含量为 1.96%。在优化条件下提取效果稳定、可重复性高。

Abstract: The extraction of alkaloids from the stem of Solanum melongena Linn. with Soxhlet method was investigated. Content of alkaloids was determined by acid-base titration. The factors of organic solvent,solid-liquid ratio,the time of extraction and the pH value of solvent were studied. The optimal extraction conditions are: 60% ethanol as solvent, the solid-liquid ratio is 1:5.5, pH value is 3, and the time of extraction is 3 h. The average content of alkaloids was 1.96%. The extaction result was stable and repetitive.