Abstract: Active substance of Lonicera maackii(Rupr.) Maxim. fresh leaves and fresh flowers were respectively extracted by Soxhlet extraction method with hexane as solvent. The volatile components were analyzed by gas chromatography-mass spectrometry(GC-MS),and their antibacterial activities were analyzed by antibacterial activity experiment. The results showed that the highest GC content compound was (-)-isolongifolol(22.63%), the second was 2,7,10-trimethyl-dodecane(10.48 %) in the fresh leaves extracts of Lonicera maackii. The highest GC content compound was 2,7,10-trimethyl-dodecane(14.16 %), and the second was the dibutyl phthalate(9.60 %) in the fresh flowers extracts of Lonicera maackii. When the mass concentration of fresh leaves extracts of Lonicera maackii was 0.19 g/mL, the inhibitory effect on Staphylococcus aureus was the highest with the antibacterial circle diameter of 13.81±1.48 mm. Lonicera maackii(Rupr.) Maxim. fresh flowers extract had good inhibition effect on Escherichia coli in the mass concentration of 0.027 5-0.22 g/mL range. According to the half inhibitory concentration of Lonicera maackii(Rupr.) Maxim. extract on three kinds of test strains, the results showed that Lonicera maackii(Rupr.) Maxim. fresh leaves extract and its flower extract of three kinds of experimental bacteria followed the inhibitory sequence of Staphylococcus aureus > Escherichia coli > Bacillus subtilis.