Abstract: The intelligent strawberry harvesting robot was designed and an automatic picking mechanism of executive body which in connection with the strawberries planted in hill-culture was developed.Based on the machine vision system of the mechanism, mature strawberries could be distinguished and located and the ripe fruits were picked by gripping and reversing peduncles so as to obtain scatheless picking. It consisted of XYZ three-axial sliding table, PLC control system based on Mitsubishi fx3 n, vision recognition system. And the control terminal and visual automatic picking algorithm was designed by C#. The robot of strawberry picking has good accuracy, high automation, strong resisting disturbance capability as well as reasonable structure. The experimental results show that the average discrimination speed is 1 s, the wrong rate of fruit is 7% and the success rate of picking can reach 90%, can satisfy the requirements of picking test for strawberry harvesting robot.