Abstract: Antibacterial properties of gleditsia saponin and camellia saponin were tested with continuous dilution method. The results showed that gleditschia saponin had some antibacterial activities against Escherichia coli(CGMCC 1.3313) and Staphylococcus aureus(CGMCC 1.2465) when the concentration is between 16 and 64 g/L. It had shown the strongest inhibitory effects against Saccharomyces cerevisiae (CICC 1855) with minimal inhibitory concentrations of 32 g/L. However, it had not any antibacterial activity against Aspergillus niger(ACCC 30557) even at high concentration. In comparison with gleditschia saponin, the antibacterial activities of camellia saponin has shown the strongest inhibitory effects against S.cerevisiae with minimal inhibitory concentration of 16 g/L and its antibacterial properties against bacterial and A.niger was the same as that of gleditschia saponin. It is feasible to use gleditschia saponin as a natural anti-bacterial additive in food industry and others after further safety evaluation.