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隐孔菌中倍半萜类化合物及其抗氧化活性研究

Antioxidant Activity of Sesquiterpenes from Cryptoporus volvatus (Peck) Hubbard

  • 摘要: 通过清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基法、2,2'-连氨-二(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)自由基法、水杨酸法和邻苯三酚自氧化法,测定隐孔菌碱提物、乙酸乙酯提取物、60%乙醇提取物和水提醇沉物4个粗提部分的抗氧化活性,结果显示:乙酸乙酯提取物清除总自由基能力最强,其对ABTS自由基半数清除率(IC50)达到(67.43±1.41)mg/L。进一步对乙酸乙酯提取物进行活性追踪分离,得到9个倍半萜类化合物:隐孔酸A三甲酯( 1 ),隐孔酸A( 2 ),隐孔酸D四甲酯( 3 ),隐孔酸D( 4 ),隐孔酸C( 5 ),隐孔酸C五甲酯( 6 ),隐孔酸E五甲酯( 7 ),隐孔酸B三甲酯( 8 )和隐孔酸B( 9 ),其中化合物 1 、 3 、 6 和 8 为首次从该种真菌中分离得到。化合物 1~9 对ABTS自由基的清除能力测定结果显示化合物 8 有较高抗氧化活性,其(IC50=(5.29±0.25)mg/L,清除能力优于阳性对照VC,VC的IC50=(5.76±0.58)mg/L。

     

    Abstract: The antioxidant activities of four extracts (alkali extracts,ethyl acetate extract,60% ethanol extract and crude polysaccharide) from Cryptoporus volvatus(Peck) Hubbard were evaluated by methods of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical,2,2'-azino-di-(3-ethyl-2,3-dihydrobenzthiazoline-6-sulfonate)(ABTS),Salicylic acid,and Fenton reaction.The results indicated that ethyl acetate extract showed the strongest free radical scavenging ability with IC50=(67.43±1.41) mg/L by the ABTS method.Further study by bioassay-guided fractionation of the ethyl acetate extract led to the isolation and identification of nine cryptoporic compounds including cryptoporic acid A trimethyl ester ( 1 ),cryptoporic acid A ( 2 ),cryptoporic acid D tetra-methyl ester ( 3 ),cryptoporic acid D ( 4 ),cryptoporic acid C ( 5 ),cryptoporic acid C pentamethyl ester ( 6 ),cryptoporic acid E pentamethyl ester ( 7 ),cryptoporic acid B trimethyl ester ( 8 ) and crytoporic acid B ( 9 ).Among them,compounds 1,3,6,8 were isolated for the first time from this fungus.The results of the ABTS scavenging ability showed that compound 8 (cryptoporic acid B trimethyl ester) has a higher antioxidant activity with IC50=(5.29±0.25) mg/L than the positive control VC with IC50=(5.76±0.58) mg/L.In addition,the structure-relationship of sesquiterpenes as antioxidant agents was analyzed.

     

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