Abstract: The classification and properties of nano-cellulose were introduced. The nano-cellulose was divided into nano-cellulose crystal(NCC), microfibrillated cellulose(MFC) and bacterial nano-cellulose(BNC). NCC has a rod-like whisker structure with high crystallinity and high mechanical properties. MFC is filamentous, with wide size range and larger aspect ratio, large specific surface area and strong hydrogen bonding ability. BNC has ultrafine network structure with high degree of chemical purity and polymerization, and strong water retention ability. The application status of nano-cellulose in paper-based reinforced materials, paper-based antimicrobial materials, paper-based filter materials, paper-based conductive materials, paper-based luminescent materials, paper-based insulating materials, paper-based hydrophobic materials and paper-based sensor materials was reviewed. The future development of nano-cellulose in paper-based functional materials was also prospected.