Synthesis and Drug Release Properties of pH-sensitive DOX@R9-PEC-NP for Drug Carrier

XU Chenshuai; WANG Fang; LI Ying; FENG Jingjing; GUO Shiwei; BAI Yujie; GUO Jianfeng

Journal of North University of China(Natural Science Edition) > 2023 > 44(3): 286-291.

XU Chenshuai, WANG Fang, LI Ying, FENG Jingjing, GUO Shiwei, BAI Yujie, GUO Jianfeng. Synthesis and Drug Release Properties of pH-sensitive DOX@R9-PEC-NP for Drug Carrier[J]. Journal of North University of China(Natural Science Edition), 2023, 44(3): 286-291.

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Synthesis and Drug Release Properties of pH-sensitive DOX@R9-PEC-NP for Drug Carrier

1. School of Chemistry and Chemical Engineering, North University of China;
2. Shanxi Sea-buckthorn Medicinal Tea Joint Innovation Research and Development Base;
3. Shanxi Provincial Key Laboratory for Chinese Lujiu Plant Extraction and Health

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Received Date: December 06, 2022

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Abstract

Abstract

Natural pectin（PEC） and polyarginine-9（R₉） were used to construct a membrane-penetrating and pH-responsive drug-loaded nanospheres（R₉-PEC-NP） for targeted drug delivery. Using PEC and R₉ as raw materials, a pectin derivative（R₉-PEC） modified with arginine was synthesized. The connection between R₉ and PEC（R₉-PEC） was characterized by element analyzer（EA） and fourier infrared spectrometer（FT-IR）. A pH-responsive nanosphere（R₉-PEC-NP） formed by CaCO₃ self-assembly in R₉-PEC solution. Scanning electron microscope（SEM） and Zeta potential and particle size analyzer were used to determine its morphology, size and potential. The loading and release of doxorubicin（DOX） by R₉-PEC-NP were analyzed with fluorescent inverted microscope and ultraviolet-visible spectroscopy（UV-Vis）. The results indicate that R₉ and PEC are successfully connected and show a regular spherical shape with the average size of R₉-PEC-NP was 215 nm, the prepared R₉-PEC-NP particles could effectively load DOX, the encapsulation efficiency is（88.70 ± 3.56）% and the drug loading is（8.15 ± 0.12） %. The results suggest that the nanospheres not only can release DOX slowly and has great pH-sensitivity, but also enable the cell-penetrating effect due to the connection of R₉, which provides potential carrier material for intelligent drug delivery systems.
- pectin,
- poly-arginine-9,
- acidity-activatable,
- drug carrier,
- smart drug delivery system

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References (20)

References

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