Abstract: Seismic safety review is an important part of sluice safety appraisal. In order to ensure the safe operation of the sluice structure,seismic safety review research is required. Taking a drop sluice as an example,based on ADINA finite element analysis software,a three-dimensional finite element model of the drop gate chamber structure is established,and the seismic response of the chamber structure is calculated and analyzed by using the mode decomposition response spectrum method,and the seismic safety evaluation of the sluice is carried out according to the calculation results. The results show that:（1）there is a tensile stress of 2.00 MPa at the intersection of the pier and the bottom plate of the gate. According to the structural mechanics method,the maximum tensile stress at this place is 4.41 MPa,which meets the safety requirements.（2）There is a large tensile stress area at the bottom of the middle span of the highway bridge of the drop sluice and part of the bottom plate of the hoist room,which is far beyond the standard value of dynamic axial tensile strength of concrete. For the sake of safety,the corresponding anti-seismic reinforcement measures should be taken.（3）The anti-sliding stability safety factor of the sluice chamber structure is greater than the standard value of sluice design code（SL 265-2016）,which meets safety requirements.（4）The anti-seismic safety of the drop gate chamber structure meets the requirements of the standard for the seismic design of hydraulic structures（GB 51247-2018）,but there are seismic defects which do not affect the overall safety,and its seismic grade is evaluated as Grade B.