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干湿环境对河岸带硝化及反硝化潜力的影响

Effect of Dry and Flooding Environment on Nitrification and Denitrification Potential in Riparian Zones

  • 摘要: 河岸带作为陆域与水域的生态交错带,硝化-反硝化作用是其氮循环的两个重要过程,探究淹水期、落干期河岸带的硝化及反硝化的潜力及其影响因素,为水体的氮污染控制提供一定的科学依据。研究选取夏家寺河岸带为研究对象,分别在淹水期和落干期收集河岸带土壤样,并通过室内培养实验测定干湿环境下河岸带的硝化、反硝化强度。结果表明:(1)无论淹水期还是落干期河岸带土壤,EC、pH、SOC、NH4+-N、NO3--N、TN含量最大值均出现表层土壤;(2)淹水期河岸带各层土壤最大硝化速率、硝化活性为(1.81、0.44、0.83)mg/(kg·d)、(86.18、22.38、40.38)%,落干期各层土壤最大硝化速率、硝化活性为(3.00、2.05、0.48)mg/(kg·d)、(138.97、96.92、24.45)%,河岸带最大硝化潜力均出现在表层土壤,落干期平均硝化潜力大于淹水期;(3)无论是淹水期还是落干期,反硝化速率随土层深度的增加而减小,表层土壤反硝化速率达到最大值,分别为69.98、63.91 mg/(kg·d),落干期河岸带平均反硝化速率24.23 mg/(kg·d)大于淹水期24.02 mg/(kg·d),淹水期反硝化活性在培养后的10 d达到最大值99.07%,落干期反硝化活性在培养后的6 d达到最大值101.91%;(4)河岸带硝化潜力、反硝化潜力与其土壤基本理化性质的相关性结果表明,淹水期硝化潜力与SOC、NH4+-N、pH含量呈显著正相关,落干期硝化潜力与TN呈显著正相关,落干期反硝化潜力与SOC、NH4+-N含量呈显著正相关,研究结果发现碳源、氮素是影响河岸带硝化反硝化潜力的主要因素。

     

    Abstract: As an ecological ecotone between land and water area,nitrification-denitrification is two important processes of nitrogen cycle in riparian zone.The potential and influencing factors of nitrification and denitrification in riparian zone during flooding period and dry period are explored.It provides a certain scientific basis for nitrogen pollution control of water body.In this study,the riparian zone of Xia jiasi was selected as the research object,the soil samples of riparian zone were collected in flooding period and dry period respectively,and the nitrification and denitrification intensity of riparian zone in dry and wet environment were measured by indoor culture experiment.The results show that:(1) the maximum content of EC,pH,SOC,NH4+-N、NO3--N and TN appear in surface soil no matter during the flooding period or during the drying period.(2) the maximum nitrification rate and nitrification activity of all layers of soil in riparian zone during flooding period were(1.81,0.44,0.83)mg/(kg·d),(86.18,22.38,40.38)%.The maximum nitrification rate and nitrification activity of each layer during the drying period were(3.00,2.05,0.48)mg/(kg·d),(138.97,96.92,24.45)%.The maximum nitrification potential of the riparian zone appeared in the topsoil,and the average nitrification potential in the dry period was higher than that in the flooding period.(3) the denitrification rate decreased with the increase of soil depth,and the denitrification rate of topsoil reached the maximum,which was(69.98,63.91)mg/(kg·d),respectively.The average denitrification rate of riparian zone in dry period24.23 mg/(kg·d)was higher than that in flooding period24.02 mg/(kg·d).The denitrification activity in flooding period reached the maximum value of 99.07%in 10 days after culture,and it reached up to 101.91%in 6 days after culture in dry period.(4) the differences in nitrification potential and denitrification potential in flooding and dry period were related to soil environmental factors.The nitrification potential in flooded period was positively correlated with SOC,NH4+-N and pH content,and nitrification potential in dry period was positively correlated with TN.There was a significant positive correlation between denitrification potential and SOC,NH4+-N content in dry period.It was found that carbon source and nitrogen were the main factors affecting nitrification and denitrification potential in riparian zone.

     

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