Abstract: The aim of this paper is to improve the understanding of water conservancy industry management and technical personnel on the revision of agricultural quota of irrigation water in Gansu Province, rationally allocate water resource, and improve the efficiency and benefit of agricultural irrigation water utilization. Based on the “Industrial Water Quota in Gansu Province（2022 Edition）”, this paper standardizes the industry code of agricultural quota of irrigation water, clarifies and supplemented the terms and definitions of agricultural quota of irrigation water, and redivided the original 5 agricultural irrigation subdivisions in Gansu Province into 12 by considering the level of agricultural economic development in the province, the trend of agricultural industrial structure adjustment, as well as regulations and requirements to meet the current and future demand of water resources. The analysis is based on the climatic resources, water resources, topography and crop planting layout of Gansu Province by using data measured from irrigation experimental stations and weather monitoring stations. This paper calculates the scientific research experimental platform of agricultural in the irrigation area, with the help of a series of meteorological, hydrological, soil and crop data, the adjustment coefficient of water source type and the conversion coefficient between the general value and the advanced value is determined, the irrigation system of the main crops under the condition of different designs of irrigation water supply guarantee rate is analyzed, at the same time the quota of irrigation water of 55 main crops in Gansu Province has been given. And we compared and analyzed the latest revision results of the state and neighboring provinces to modify it again. The results show that the coverage, rational, advanced and progressive of the conclusion is improved obviously. However, it is still necessary to revise the result at the right moment because of the dynamic, certain, different, mandatory and other characteristics of agricultural quota of irrigation water and the current limitation and transformation of the layout of agriculture.