Abstract: It is of great significance for the ecological protection and high-quality development of the Yellow River Basin to explore the coupling coordination relationship between ecosystem services and human activity intensity in the Yellow River Basin and its influencing factors.Unfortunately, there is a relative lack of research on integrating the human activity intensity into the correlation analysis of ecosystem services to explore the coupling coordination between the two, especially in the Yellow River Basin. In this paper, based on multi-source data such as remote sensing and socio-economic statistics, the spatial and temporal variation characteristics of ecosystem service values and human activity intensity in the Yellow River Basin from 2000-2018 are assessed by using the modified equivalent factor method and the human activity intensity method. Then the spatial and temporal evolution of the coupling coordination relationship between them are revealed by using the coupling coordination degree model. Finally, the dominant driving factors affecting the change of the coupling coordination relationship between ecosystem service values and human activity intensity are discussed by using the geographical detector model. The results show that:（1） The ecosystem service value in the Yellow River Basin showed an increasing trend from 2000-2018, with an increase of 8.492 7 billion yuan during the whole study period, and showed a spatial distribution pattern of low ecosystem service value in the eastern municipalities and high ecosystem service value in the central and western prefecture-level cities of the Yellow River Basin.（2） Most of municipalities in the Yellow River Basin showed an increasing trend of human activity intensity from 2000-2018, indicating that the human activities in the Yellow River Basin are increasingly interfering with the ecosystem, and showed a spatial pattern of decreasing human activity intensity from southeast to northwest.（3） The coupling degree between ecosystem service value and human activity intensity in most municipalities of the Yellow River Basin was at the stages of friction and high level of coupling, and the coupling coordination degree was mostly between barely coordinated and basically coordinated.（4） The results of geographical exploration showed that economic density, construction land ratio, rainfall and NDVI were the dominant influencing factors driving the changes of coupling coordination degree between ecosystem service value and human activity intensity in the Yellow River Basin. The results not only enrich the knowledge of the relationship between ecosystem services and human activities, but also provide an important scientific basis for the sustainable development of the ecosystem in the Yellow River Basin.