Abstract: The multi-hole inverted siphon project has the advantages of the large flow rate accommodation and flexible scheduling. However,the non-uniform flow distribution among the holes directly affects the flow capacity and the flow regime,and even the large water level fluctuations,which can induce engineering vibration. Taking a three-hole inverted siphon project of a water transmission pipeline in a plain area as an example,this paper emphatically analyzes the distribution of the flow regime in inlet and outlet and the characteristics of water level fluctuation under the condition of multi-hole scheduling operation. The optimization plans are proposed to adjust the flow regime in the inlet and reduce the water level fluctuation. The influence of the emergency closing of the valve and the variation of roughness on the operation of inverted siphon project is discussed. The results can provide a reference for similar projects.