Abstract: In order to solve the problem of optimal operation for the tidal pumping station under the water supply mode,this paper proposes a real-time optimization strategy. First,the pump characteristic curves are converted into interpolable data sets,and a mathematical model of the pump is constructed by using a three-dimensional interpolation method. Then the optimization period is discretely processed to construct a state matrix,which expresses the operating conditions of the pumping station at the target period. Finally,the length of the operating period,the number of working pumps,and the blade angle are limited to the feasible solution set,and the genetic algorithm is used to find the optimal solution. The strategy supports the free selection of optimal time periods and the free definition of feasible solution sets,which fit the actual needs of engineering operation and scheduling. Calculation examples show that the method is fast,and the results are reasonable,which can meet the needs of tidal pumping stations’ real-time optimal operation.