Abstract: Isotopes are adopted to label different water bodies to test and analyze the stable isotopes in the Hanbei River Basin. The transformation relationship and conversion ratio of atmospheric precipitation,rivers,soil water and groundwater bodies in the study area are explored to provide technical support for the sustainable management of water resources in the study area. The results show that the regional precipitation line is δD=8.44δ¹⁸O+7.88. Compared with the global precipitation line,the slope and intercept of the precipitation line in the Hanbei Basin are offset to a certain extent,and precipitation is obviously affected by evaporation. The results show that the proportion of atmospheric precipitation,soil water and groundwater in the river water of Hanbei Basin is 38.2%,15.8% and 19.3%,respectively.