Abstract: Aiming at the problem that some current kiwi pollen separators have, such as pollen bonding when they are used in Guizhou that cannot be effectively separated, the article proposes a method to solve the problem of pollen separation based on the principle of semiconductor refrigeration. The freezing model before pollen separation was established, and COMSOL was used to simulate the temperature field of the freezing model. Through the simulation analysis of the uniformity of the temperature field of the freezer when the cooling fins are in different positions, it is determined that the temperature field in the box is the most suitable for freezing before pollen separation when the C is empty. The result was applied to actual kiwi pollen separation. The simulation results show that the freezing box can quickly realize the temperature change from 293 K to 258 K, which meets the actual production requirements; thus, the uniformity of the temperature field is significantly improved. The results of the freezing separation test of kiwi flowers showed that the separation effect and pollen extraction rate after freezing at-21 ℃ were higher than the separation and purification results of unfrozen and frozen at-18 ℃. When pollen is frozen at-21 ℃, the powder extraction rate is 3.9‰, which is similar to the amount of pollen extracted by manual separation, thus, realizing the efficient separation and extraction of Kiwi fruit pollen.