Abstract: In order to estimate the pollution amount of livestock and poultry breeding and evaluate the pollution load of livestock and poultry breeding in Guizhou, and provide a basis for the pollution control of livestock and poultry breeding, based on the statistical data of livestock and poultry breeding quantity and common cultivated land area from 2007 to 2020 in Guizhou province and other cities and prefectures, the early warning study on livestock and poultry breeding pollution load was carried out by means of the pollutant discharge coefficient method and land bearing method.The results showed that the total feces and urine of livestock and poultry in each city and prefecture was 100 248.79 kt, which was 1.23 times of industrial solid waste, among of which cattle produced the most feces and urine, followed by pigs, sheep and poultry. The total amount of pollutants discharged by livestock and poultry manure in each city and prefecture was 5 184.87 kt, among which the emissions of COD and BOD were large, while the emissions of TP, TN and NH₃-N were small. The total loss of livestock and poultry manure pollutants was 630.41 kt, in which the discharge of COD accounted was the highest proportion. Except Guiyang City, the pig manure equivalent, N and P load of all cities and prefectures had exceeded the standard, and livestock and poultry breeding had caused great pollution risk to agricultural cultivated land in Guizhou, and the pollution severity was in the order of Anshun City ＞ Qiannan Prefecture ＞ Tongren City ＞ Qianxinan Prefecture ＞ Qiandongnan Prefecture ＞ Liupanshui City ＞ Zunyi City ＞ Bijie City. At last, on base of study conclusion, the relevant suggestions are put forward, such as rational distribution of livestock and poultry breeding scale, strengthening the supervision of sewage discharge system, implementing subsidy policies, promoting clean livestock and poultry breeding and improving feed conversion ratio.