Abstract: “Huxiang F2” Shiitake spawn sticks during the color change period upon debagging were taken as the test object, and their physical and mechanical properties were investigated. The density of Shiitake spawn sticks was measured using the sand discharge method, yielding a value of 0.64 g/cm~3. The dynamic friction coefficients between spawn sticks and spawn bags, as well as between spawn sticks and 65Mn spring steel, were determined to be 0.485 and 0.584, respectively, employing the slope method. The attachment strength and strain tear-off force averaged at 1.01 kPa and 25.69 N, respectively. A radial compression test was performed on the Shiitake spawn sticks using an electronic universal testing machine. Combining the experimental results with theoretical calculations, when the loading speed of the spawn sticks was 10 mm/min, the average radial compressive strength of the rods was found to be 44.86 kPa, with corresponding average values of elastic modulus and Poisson’s ratio of 0.48 MPa and 0.17, respectively. These research findings establish a fundamental theoretical foundation for the design of key components and the determination of operating parameters for Shiitake spawn stick de-bagging equipment.